- Titel
- Development of an automated platform for monitoring microfluidic reactors through multi- reactor integration and online (chip-)LC/MS- detection
- Zitierfähige Url:
- https://nbn-resolving.org/urn:nbn:de:bsz:15-qucosa2-1021367
- Quellenangabe
- Reaction Chemistry & Engineering
Erscheinungsjahr: 2024
Jahrgang: 9
Heft: 7
Seiten: 1739-1750
E-ISSN: 2058-9883 - Erstveröffentlichung
- 2024
- Abstract (EN)
- This work presents a novel microfluidic screening setup with real-time analytics for investigating reactions with immobilised biocatalysts. The setup combines microreactor technology, multi-reactor integration, and online (chip-)LC/MS analysis in a sequential automated workflow. We utilized in-house manufactured fused-silica glass chips as reusable packed-bed microreactors interconnected as individual tube reactors. The potential of this setup was showcased by conducting and optimising a biocatalytic aromatic bromination reaction as the first proof of concept using immobilised vanadium-dependent haloperoxidase from Curvularia inaequalis (CiVHPO). The fusion of a HaloTag™ to CiVHPO was used for efficient and mild covalent linkage of the enzyme onto chloroalkane-functionalized particles. Then, the biotransformation was continuously monitored with automated LC/MS data acquisition in a data-rich manner. By further developing the automation principle, it was possible to sequentially screen multiple different connected packed-bed microreactors for reaction optimization while using only miniature amounts of reactants and biocatalyst. Finally, we present a fast and modular chipHPLC solution for online analysis to reduce the overall solvent consumption by over 80%. We established a modern microfluidic platform for real-time reaction monitoring and evaluation of biocatalytic reactions through automation of the reactant feed integration, flexible microreactor selection, and online LC/MS analysis.
- Andere Ausgabe
- Erstveröffentlichung
DOI: 10.1039/d4re00004h - Freie Schlagwörter (EN)
- online (chip-)LC/MS- detection, automated platform, monitoring microfluidic reactors, multi- reactor integration
- Klassifikation (DDC)
- 540
- Verlag
- The Royal Society of Chemistry, London
- Förder- / Projektangaben
- Deutsche Forschungsgemeinschaft (DFG)
ID: GU 1134/3 - Version / Begutachtungsstatus
- publizierte Version / Verlagsversion
- URN Qucosa
- urn:nbn:de:bsz:15-qucosa2-1021367
- Veröffentlichungsdatum Qucosa
- 06.02.2026
- Dokumenttyp
- Artikel
- Sprache des Dokumentes
- Englisch
- Lizenz / Rechtehinweis
CC BY 4.0