- Titel
- Dynamic interplay between target search and recognition for a Type I CRISPR-Cas system
- Zitierfähige Url:
- https://nbn-resolving.org/urn:nbn:de:bsz:15-qucosa2-1043217
- Quellenangabe
- Nature Communications
Erscheinungsjahr: 2023
Jahrgang: 14
Heft: 1
E-ISSN: 2041-1723
Artikelnummer: 3654 - Erstveröffentlichung
- 2023
- Abstract (EN)
- CRISPR-Cas effector complexes enable the defense against foreign nucleic acids and have recently been exploited as molecular tools for precise genome editing at a target locus. To bind and cleave their target, the CRISPR-Cas effectors have to interrogate the entire genome for the presence of a matching sequence. Here we dissect the target search and recognition process of the Type I CRISPR-Cas complex Cascade by simultaneously monitoring DNA binding and R-loop formation by the complex. We directly quantify the effect of DNA supercoiling on the target recognition probability and demonstrate that Cascade uses facilitated diffusion for its target search. We show that target search and target recognition are tightly linked and that DNA supercoiling and limited 1D diffusion need to be considered when understanding target recognition and target search by CRISPR-Cas enzymes and engineering more efficient and precise variants.
- Andere Ausgabe
- Erstveröffentlichung
DOI: 10.1038/s41467-023-38790-1 - Freie Schlagwörter (EN)
- Type I CRISPR-Cas system, Dynamic interplay, target search and recognition
- Klassifikation (DDC)
- 500
- Verlag
- Nature Publishing Group UK, London
- Förder- / Projektangaben
- Europäischer Forschungsrat (ERC)
ID: GA 724863 - Deutsche Forschungsgemeinschaft (DFG)
ID: SE 1646/9-1 - Version / Begutachtungsstatus
- publizierte Version / Verlagsversion
- URN Qucosa
- urn:nbn:de:bsz:15-qucosa2-1043217
- Veröffentlichungsdatum Qucosa
- 05.05.2026
- Dokumenttyp
- Artikel
- Sprache des Dokumentes
- Englisch
- Lizenz / Rechtehinweis
CC BY 4.0