- AutorIn
- Christoph Ottenheim
- Katharina A. Werner
- Wolfgang Zimmermann
- Jin Chua Wu
- Titel
- Improved endoxylanase production and colony morphology of Aspergillus niger DSM 26641 by g-ray induced mutagenesis
- Zitierfähige Url:
- https://nbn-resolving.org/urn:nbn:de:bsz:15-qucosa2-168540
- Quellenangabe
- Biochemical Engineering Journal
Erscheinungsjahr: 2015
Jahrgang: 94
Seiten: 9-14
DOI: 10.1016/j.bej.2014.10.020 - Erstveröffentlichung
- 2015
- Abstract (EN)
- Aspergillus niger DSM 26641 was exposed to 60Co g-radiation to enhance the b-1,4-endoxylanase activity, restrict colony growth and improve robustness of pellets. The first promising mutant obtained after g-radiation of the fungal spores at 50-2000 Gy showed a restricted colony growth and an 82% enhancement in b-1,4-endoxylanase activity. The mutant was subjected to a second round of g-radiation at 1400 Gy generating a mutant with double the b-1,4-endoxylanase activity compared to the native strain. The selected final mutant, deposited as Aspergillus niger DSM 28712, showed a maximal saccharification activity of 26 U·ml-1 on xylan based broth, 48 U·ml-1 on lignocellulose hydrolysate and 375 U·ml-1 on lignocellulose hydrolysate supplemented with yeast extract and mineral salts.
- Andere Ausgabe
- Improved endoxylanase production and colony morphology of Aspergillus niger DSM 26641 by g-ray induced mutagenesis
DOI: 10.1016/j.bej.2014.10.020 - Freie Schlagwörter (EN)
- Filamentous Fungi, Radiation Mutagenesis, Enzymes, Microbial Growth, Optimisation
- Klassifikation (DDC)
- 572
- Herausgeber (Institution)
- Universität Leipzig, Leipzig
- Version / Begutachtungsstatus
- angenommene Version / Postprint / Autorenversion
- URN Qucosa
- urn:nbn:de:bsz:15-qucosa2-168540
- Veröffentlichungsdatum Qucosa
- 01.12.2017
- Dokumenttyp
- Artikel
- Sprache des Dokumentes
- Englisch