LIST OF ABBREVIATIONS........................................................................................ 5
INDEX OF FIGURES..................................................................................................7
INDEX OF TABLES.....................................................................................................8
SUMMARY.................................................................................................................9

1	INTRODUCTION..............................................................................................11
1.1	Cutaneous wound healing...............................................................................11
1.2	Phases of wound healing................................................................................11
1.2.01	Inflammation............................................................................................11
1.2.02	Proliferation..............................................................................................13
1.2.03	Remodeling..............................................................................................16
1.3	Wnt-signaling...................................................................................................17
1.3.01	Wnt-ligands biogenesis and receptors....................................................17
1.3.02	Canonical and non canonical Wnt signaling...........................................20
1.3.03	Canonical Wnt/ß catenin signaling..........................................................20
1.4	Wound healing and canonical Wnt/ß catenin signaling...................................21

2	AIMS OF THE STUDY......................................................................................25

3	MATERIALS AND EXPERIMENTAL PROCEDURES........................................27
3.1	MATERIALS.....................................................................................................27
3.1.01	Instruments..............................................................................................27
3.1.02	Software..................................................................................................27
3.1.03	Websites..................................................................................................28
3.1.04	Consumables...........................................................................................28
3.1.05	Media and Buffers...................................................................................29
3.1.06	Reagents..................................................................................................29
3.1.07	Stimulation reagents................................................................................30
3.1.08	Anesthesia and analgesia used for the full-thickness skin wound model.......................................................................................................................30
3.1.09	Kits...........................................................................................................30
3.1.10	Quantitative reverse-transcription PCR primers......................................31

3.2	EXPERIMENTAL PROCEDURES.....................................................................32
3.2.01	Mice.........................................................................................................32
3.2.02	Cell Lines.................................................................................................32
3.2.03	Cell counting with trypan blue stain and hemocytometer method.........32
3.2.04	Preparation of murine H133WT immortalized primary keratinocytes cultures....................................................................................................................33
3.2.05	Preparation of RAW264.7 murine macrophages cultures.......................33
3.2.06	Preparation of primary murine fibroblasts cultures.................................33
3.2.07	Type 1 and type 2 immunity cell stimulation...........................................34
3.2.08	Cell RNA isolation....................................................................................34
3.2.09	Mice wounding........................................................................................35
3.2.10	Whole skin tissue isolation......................................................................36
3.2.11	Epidermal and dermal tissue isolation....................................................36
3.2.12	Tissue RNA isolation...............................................................................36
3.2.13	cDNA synthesis.......................................................................................36
3.2.14	Quantitative Real-Time PCR (qPCR).......................................................37
3.2.15	Statistical analysis...................................................................................37

4	EXPERIMENTAL RESULTS..............................................................................39
4.1	Canonical Wnt/ß-catenin signaling activity in different skin cell populations under type 1 and type 2 immunity cytokines stimulation in vitro............................39
4.2	Canonical Wnt/ß-catenin signaling activity under type 1 and type 2 immunity conditions during wound healing in vivo.................................................................42
4.3	Wnt-ligands expression in different skin cell populations under type 1 and type 2 immunity cytokines stimulation in vitro.........................................................44
4.4	Wnt ligands expression in epidermal and dermal fraction under type 1 and type 2 immunity conditions in vivo..........................................................................51

5	DISCUSSION...................................................................................................53
6	CONCLUSIONS...............................................................................................59
7	PERSPECTIVES...............................................................................................61
8	REFERENCES..................................................................................................63
9	APPENDIX.......................................................................................................71
9.1	Supplementary results.....................................................................................71
9.2	Erklärung über die eigenständige Abfassung der Arbeit.................................73
9.3	Curriculum Vitae..............................................................................................75
9.4	Acknowledgments...........................................................................................79