- AutorIn
- Markus Friedemann
- Liv Kalbitzer
- Sandra Franz
- Stephanie Moeller
- Matthias Schnabelrauch
- Jan-Christoph Simon
- Tilo Pompe
- Katja Franke
- Titel
- Instructing human macrophage polarization by stiffness and glycosaminoglycan functionalization in 3D collagen networks
- Zitierfähige Url:
- https://nbn-resolving.org/urn:nbn:de:bsz:15-qucosa2-365391
- Quellenangabe
- Advanced Healthcare Materials Erscheinungsort: Weinheim
Verlag: Wiley-VCH Verlag
Erscheinungsjahr: 2017
Jahrgang: 6
Heft: 7
E-ISSN: 2192-2659
DOI: 10.1002/adhm.201600967
Artikelnummer: 1600967 - Erstveröffentlichung
- 2017
- Abstract (EN)
- Dynamic alterations of composition and mechanics of the extracellular matrix (ECM) are suggested to modulate cellular behavior including plasticity of macrophages (MPhs) during wound healing. In this study, engineered 3D fibrillar matrices based on naturally occurring biopolymers (collagen I, glycosaminoglycans (GAGs)) were used to mimic matrix stiffening as well as modification by sulfated and non-sulfated GAGs at different stages of wound healing. Human MPhs were found to sensitively respond to these microenvironmental cues in terms of polarization towards pro-inflammatory or wound healing phenotypes over 6 days in vitro. MPhs exhibited a wound healing phenotype in stiffer matrices as determined by protein and gene expression of relevant cytokines (IL10, IL12, TNF). Presence of sulfated and non-sulfated GAGs inhibited this polarization effect. Furthermore, control experiments on 2D matrices stressed the relevance of using stiffness-controlled 3D matrices, as MPhs showed a reciprocal polarization behavior depending on GAG presence. Hence, the results indicate a strong influence of dimensionality, stiffness, and GAG presence of the biomaterial scaffold on MPh polarization and emphasize the need for matrices closely mimicking the 3D in vivo context with a variable stiffness and GAG composition in in vitro studies.
- Freie Schlagwörter (DE)
- Menschliche Makrophagen, Extrazelluläre Matrix, Kollagen
- Freie Schlagwörter (EN)
- Human macrophages, Extracellular matrix, Collagen, Glycosaminoglycan, Stiffness
- Klassifikation (DDC)
- 572
- Version / Begutachtungsstatus
- angenommene Version / Postprint / Autorenversion
- URN Qucosa
- urn:nbn:de:bsz:15-qucosa2-365391
- Veröffentlichungsdatum Qucosa
- 16.12.2019
- Dokumenttyp
- Artikel
- Sprache des Dokumentes
- Englisch