- Titel
- Various cross-linking methods inhibit the collagenase I degradation of rabbit scleral tissue
- Zitierfähige Url:
- https://nbn-resolving.org/urn:nbn:de:bsz:15-qucosa2-780203
- Quellenangabe
- BMC Ophthalmology
Erscheinungsjahr: 2020
Jahrgang: 20
E-ISSN: 1471-2415
Artikelnummer: 488 - Erstveröffentlichung
- 2020
- Abstract (EN)
- Background: Collagen cross-linking of the sclera is a promising approach to strengthen scleral rigidity and thus to inhibit eye growth in progressive myopia. Additionally, cross-linking might inhibit degrading processes in idiopathic melting or in ocular inflammatory diseases of the sclera. Different cross-linking treatments were tested to increase resistance to enzymatic degradation of the rabbit sclera. Methods: Scleral patches from rabbit eyes were cross-linked using paraformaldehyde, glutaraldehyde or riboflavin combined with UV-A-light or with blue light. The patches were incubated with collagenase I (MMP1) for various durations up to 24 h to elucidate differences in scleral resistance to enzymatic degradation. Degraded protein components in the supernatant were detected and quantified using measurements of Fluoraldehyde o-Phthaldialdehyde (OPA) fluorescence . Results: All cross-linking methods reduced the enzymatic degradation of rabbit scleral tissue by MMP1. Incubation with glutaraldehyde (1%) and paraformaldehyde (4%) caused nearly a complete inhibition of enzymatic degradation (down to 7% ± 2.8 of digested protein compared to control). Cross-linking with riboflavin/UV-A-light reduced the degradation by MMP1 to 62% ± 12.7 after 24 h. Cross-linking with riboflavin/blue light reduced the degradation by MMP1 to 77% ± 13.5 after 24 h. No significant differences could be detected comparing different light intensities, light exposure times or riboflavin concentrations. Conclusions: The application of all cross-linking methods increased the resistance of rabbit scleral tissue to MMP1-degradation. Especially, gentle cross-linking with riboflavin and UV-A or blue light might be a clinical approach in future.
- Andere Ausgabe
- Link zur Originalveröffentlichung in BMC Ophthalmology
DOI: 10.1186/s12886-020-01751-z
Link: https://doi.org/10.1186/s12886-020-01751-z - Freie Schlagwörter (EN)
- Scleral cross-linking, Rabbit sclera, Glutaraldehyde, Paraformaldehyde, Riboflavin/UV-A-light, Riboflavin/blue light
- Klassifikation (DDC)
- 610
- 000
- Verlag
- BMC, London
- Version / Begutachtungsstatus
- publizierte Version / Verlagsversion
- URN Qucosa
- urn:nbn:de:bsz:15-qucosa2-780203
- Veröffentlichungsdatum Qucosa
- 11.02.2022
- Dokumenttyp
- Artikel
- Sprache des Dokumentes
- Englisch
- Lizenz / Rechtehinweis
CC BY 4.0