- Titel
- Electrochemical Microwell Plate to Study Electroactive Microorganisms in Parallel and Real-Time
- Zitierfähige Url:
- https://nbn-resolving.org/urn:nbn:de:bsz:15-qucosa2-845003
- Quellenangabe
- Frontiers in Bioengineering and Biotechnology
Erscheinungsjahr: 2021
Jahrgang: 9
E-ISSN: 2296-4185
Artikelnummer: 821734 - Erstveröffentlichung
- 2021
- Abstract (EN)
- Microbial resource mining of electroactive microorganism (EAM) is currently methodically hampered due to unavailable electrochemical screening tools. Here, we introduce an electrochemical microwell plate (ec-MP) composed of a 96 electrochemical deepwell plate and a recently developed 96-channel multipotentiostat. Using the ec-MP we investigated the electrochemical and metabolic properties of the EAM models Shewanella oneidensis and Geobacter sulfurreducens with acetate and lactate as electron donor combined with an individual genetic analysis of each well. Electrochemical cultivation of pure cultures achieved maximumcurrent densities (jmax) and coulombic efficiencies (CE) that were well in line with literature data. The co-cultivation of S. oneidensis and G. sulfurreducens led to an increased current density of jmax of 88.57 ± 14.04 μA cm−2 (lactate) and jmax of 99.36 ± 19.12 μA cm−2 (lactate and acetate). Further, a decreased time period of reaching jmax and biphasic current production was revealed and the microbial electrochemical performance could be linked to the shift in the relative abundance.
- Andere Ausgabe
- Link zur Erstveröffentlichung
Link: https://doi.org/10.3389/fbioe.2021.821734 - Freie Schlagwörter (EN)
- microbial electrochemical technology, electroactive microorganisms, microbial ecology, multipotentiostat, microbial resource mining
- Klassifikation (DDC)
- 570
- Verlag
- Frontiers Research Foundation, Lausanne
- Version / Begutachtungsstatus
- publizierte Version / Verlagsversion
- URN Qucosa
- urn:nbn:de:bsz:15-qucosa2-845003
- Veröffentlichungsdatum Qucosa
- 03.04.2023
- Dokumenttyp
- Artikel
- Sprache des Dokumentes
- Englisch
- Lizenz / Rechtehinweis
CC BY 4.0