- Titel
- Influence of Culture Conditions on Ex Vivo Expansion of T Lymphocytes and Their Function for Therapy
- Untertitel
- Current Insights and Open Questions
- Zitierfähige Url:
- https://nbn-resolving.org/urn:nbn:de:bsz:15-qucosa2-875169
- Quellenangabe
- Frontiers in Bioengineering and Biotechnology
Erscheinungsjahr: 2022
Jahrgang: 10
Artikelnummer: 886637 - Erstveröffentlichung
- 2022
- Abstract (EN)
- Ex vivo expansion of T lymphocytes is a central process in the generation of cellular therapies targeted at tumors and other disease-relevant structures,which currently cannot be reached by established pharmaceuticals. The influence of culture conditions on T cell functions is, however, incompletely understood. In clinical applications of ex vivo expanded T cells, so far, a relatively classical standard cell culture methodology has been established. The expanded cells have been characterized in both preclinical models and clinical studies mainly using a therapeutic endpoint, for example antitumor response and cytotoxic function against cellular targets, whereas the influence of manipulations of T cells ex vivo including transduction and culture expansion has been studied to a much lesser detail, or in many contexts remains unknown. This includes the circulation behavior of expanded T cells after intravenous application, their intracellular metabolism and signal transduction, and their cytoskeletal (re)organization or their adhesion, migration, and subsequent intra-tissue differentiation. This review aims to provide an overview of established T cell expansion methodologies and address unanswered questions relating in vivo interaction of ex vivo expanded T cells for cellular therapy.
- Andere Ausgabe
- Link zur Erstveröffentlichung
Link: https://doi.org/10.3389/fbioe.2022.886637 - Freie Schlagwörter (EN)
- T lymphocyte (T-cell), ex vivo expansion, CAR T cell, cell culture conditions, homing
- Klassifikation (DDC)
- 610
- Verlag
- Frontiers Media S.A., Lausanne
- Version / Begutachtungsstatus
- publizierte Version / Verlagsversion
- URN Qucosa
- urn:nbn:de:bsz:15-qucosa2-875169
- Veröffentlichungsdatum Qucosa
- 20.10.2023
- Dokumenttyp
- Artikel
- Sprache des Dokumentes
- Englisch
- Lizenz / Rechtehinweis
CC BY 4.0