- AutorIn
- Liv Kalbitzer
- Tilo Pompe
- Titel
- Fibril growth kinetics link buffer conditions and topology of 3D collagen I networks
- Zitierfähige Url:
- https://nbn-resolving.org/urn:nbn:de:bsz:15-qucosa2-331144
- Quellenangabe
- Acta Biomaterialia Erscheinungsort: Amsterdam
Verlag: Elsevier
Erscheinungsjahr: 2018
Jahrgang: 67
Heft: February 2018
Seiten: 206-214
ISSN: 1742-7061 - Erstveröffentlichung
- 2018
- DOI
- https://doi.org/10.1016/j.actbio.2017.11.051
- Abstract (EN)
- Three-dimensional fibrillar networks reconstituted from collagen I are widely used as biomimetic scaffolds for in vitro and in vivo cell studies. Various physicochemical parameters of buffer conditions for in vitro fibril formation are well known, including pH-value, ion concentrations and temperature. However, there is a lack of a detailed understanding of reconstituting well-defined 3D network topologies, which is required to mimic specific properties of the native extracellular matrix. We screened a wide range of relevant physicochemical buffer conditions and characterized the topology of the reconstituted 3D networks in terms of mean pore size and fibril diameter. A congruent analysis of fibril formation kinetics by turbidimetry revealed the adjustment of the lateral growth phase of fibrils by buffer conditions to be key in the determination of pore size and fibril diameter of the networks. Although the kinetics of nucleation and linear growth phase were affected by buffer conditions as well, network topology was independent of those two growth phases. Overall, the results of our study provide necessary insights into how to engineer 3D collagen matrices with an independent control over topology parameters, in order to mimic in vivo tissues in in vitro experiments and tissue engineering applications.
- Andere Ausgabe
- Link zur Originalpublikation
Link: https://www.sciencedirect.com/science/article/pii/S1742706117307481
DOI: 10.1016/j.actbio.2017.11.051 - Freie Schlagwörter (EN)
- Collagen I, 3D matrices, Pore size, Fibril diameter, Turbidimetry
- Klassifikation (DDC)
- 572
- Version / Begutachtungsstatus
- angenommene Version / Postprint / Autorenversion
- URN Qucosa
- urn:nbn:de:bsz:15-qucosa2-331144
- Veröffentlichungsdatum Qucosa
- 07.02.2019
- Dokumenttyp
- Artikel
- Sprache des Dokumentes
- Englisch