- AutorIn
- Jiranuwat Sapudom
- Stefan Rubner
- Steve Martin
- Tony Kurth
- Stefanie Riedel
- Claudia Mierke
- Tilo Pompe
- Titel
- Space constraints govern fate of hematopoietic stem and progenitor cells in vitro
- Zitierfähige Url:
- https://nbn-resolving.org/urn:nbn:de:bsz:15-qucosa2-331297
- Quellenangabe
- Biomaterials Erscheinungsort: Amsterdam
Verlag: Elsevier
Erscheinungsjahr: 2015
Jahrgang: 53
Heft: June 2015
Seiten: 709-715
ISSN: 0142-9612 - Erstveröffentlichung
- 2015
- DOI
- https://doi.org/10.1016/j.biomaterials.2015.02.095
- Abstract (EN)
- Deciphering exogenous cues that determine stem cell fate decisions is a persisting challenge of cell biology and bioengineering. In an effort to unravel the role of spatial constraints in the cell-instructive characteristics of bone marrow microenvironments, murine hematopoietic stem and progenitor cells (HSPC) were exposed to fibronectin-coated microcavities in vitro. Microcavity sizes were chosen to allow for the inclusion of either individual or multiple cells. Repopulation experiments using lethally irradiated mice showed that the maintenance of functional HSPC in culture critically depends on cavity dimensions. Short-term repopulating hematopoietic stem cells (ST-HSC) were found to be best supported within single-cell sized compartments while long-term repopulating HSC (LT-HSC) were maintained within both cavity sizes. In sum, the reported data reveal spatial restriction to be a simple but powerful means for directing HSPC fate ex vivo.
- Andere Ausgabe
- Link zur Originalpublikation
Link: https://www.sciencedirect.com/science/article/pii/S0142961215002331
DOI: 10.1016/j.biomaterials.2015.02.095 - Freie Schlagwörter (EN)
- ECM, In vivo test, Microstructure, Stem cell
- Klassifikation (DDC)
- 572
- Version / Begutachtungsstatus
- angenommene Version / Postprint / Autorenversion
- URN Qucosa
- urn:nbn:de:bsz:15-qucosa2-331297
- Veröffentlichungsdatum Qucosa
- 08.02.2019
- Dokumenttyp
- Artikel
- Sprache des Dokumentes
- Englisch